|ELISA (ELISA)||8 µg/ml|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||1.0 ug/ml|
|Western Blot (WB)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant OSCP and PDH E1-alpha|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
For positive control, use isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E1-alpha polypeptide 1; mitochondrial; PDHA; PDHCE1A; PDHE1-A type I; PHE1A; pyruvate dehydrogenase complex; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 component alpha subunit; pyruvate dehydrogenase E1 component subunit alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit; somatic form
Gene Aliases: BOS_26312; PDHA; Pdha-1; PDHA1; PDHAD; PDHCE1A; PHE1A
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