|Western Blot (WB)||2 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to the amino acids CIQEVWRQRYQSHPDAAVQ of human PDK1|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial; mitochondrial pyruvate dehydrogenase; PkB kinase like gene 1; pyruvate dehydrogenase kinase
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) O15530
Entrez Gene ID: (Human) 5170
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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