|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near C-terminus of human PIM2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is B cell lymphoma.
PIM-2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM-1 and PIM-3. Expression of this protein is regulated by hematopoietic cytokines. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM-2 in proliferating cells as well as during meiosis. PIM-2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumors including lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: pim-2 oncogene; Pim-2h; proto-oncogene Pim-2 (serine threonine kinase); proto-oncogene Pim-2 serine threonine kinase; serine/threonine protein kinase pim-2; Serine/threonine-protein kinase pim-2
Gene Aliases: PIM2
UniProt ID: (Human) Q9P1W9
Entrez Gene ID: (Human) 11040
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