|Western Blot (WB)||1:1000-1:10,000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 273 of PKC theta (Uniprot ID#Q04759)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-34752 targets PKC theta in WB applications and shows reactivity with Human samples.
The PA5-34752 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 273 of PKC theta (Uniprot ID#Q04759).
Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: nPKC-theta; PRKCT; Protein kinase C theta type; protein kinase C, theta
Gene Aliases: nPKC-theta; PRKCQ; PRKCT
UniProt ID: (Human) Q04759
Entrez Gene ID: (Human) 5588
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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