|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding human placental alkaline phosphatase.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is seminoma or placenta tissue.
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placents during the 3rd trimester of gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alkaline phosphatase; Alkaline phosphatase Regan isozyme; Alkaline phosphatase, placental type; alkaline phosphomonoesterase; glycerophosphatase; PALP; Placental alkaline phosphatase 1; placental type; PLAP; PLAP-1; Regan isozyme
Gene Aliases: ALP; ALPP; PALP; PLAP; PLAP-1
UniProt ID: (Human) P05187
Entrez Gene ID: (Human) 250
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