|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 16 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunoprecipitation (IP)||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa/IgG2b|
|Immunogen||A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein(|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
33-1700 contains a cocktail of monoclonal mouse PLK1 containing clone PL6, isotype IgG1, kappa and clone PY20, isotype IgG2b.
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; Plx1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13
Gene Aliases: PLK; PLK1; plx1; STPK13
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