|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 161 and 470 of Human PLK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28023 targets PLK1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28023 immunogen is recombinant fragment corresponding to a region within amino acids 161 and 470 of Human PLK1.
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cell cycle regulated protein kinase; PLK; PLK-1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Human) P53350
Entrez Gene ID: (Human) 5347
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