|Flow Cytometry (Flow)||1ug/test|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:5000|
|Western Blot (WB)||See 47 publications below|
|Immunohistochemistry (IHC)||See 7 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Immunoprecipitation (IP)||See 4 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Amphibian, Dog, Chicken, Cat, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat|
|Published species reactivity||Dog , Rabbit , Rat , Pig , Non-human primate , Amphibian , Bovine , Hamster , Cat , Mouse , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human erythrocyte calcium ATPase.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.
MA3-914 has been successfully used in Western blot, FACS, immunohistochemistry, immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~140 kDa protein representing PMCA ATPase and bands at 95kDa and 180 kDa which probably represent products of aggregation and/or natural proteolytic products of the pump from rat liver membrane preparations. Immunohistochemical staining of PMCA ATPase with MA3-914 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.
MA3-914 can be used on both frozen and formalin-fixed paraffin-embedded tissues.
The MA3-914 antigen is purified human erythrocyte calcium ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase plasma membrane Ca2+ transporting 1; ATPase, Ca++ transporting, plasma membrane 1; ATPase, Ca++ transporting, plasma membrane 2; ATPase, Ca++ transporting, plasma membrane 3; ATPase, Ca++ transporting, plasma membrane 4; Ca2+/Mg2+ ATPase; cilia and flagella associated protein 39; deaf waddler; Matrix-remodeling-associated protein 1; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma Membrane Calcium Pump ATPase; Plasma membrane calcium pump isoform 1; Plasma membrane calcium pump isoform 2; Plasma membrane calcium pump isoform 3; Plasma membrane calcium pump isoform 4; Plasma membrane calcium-transporting ATPase 1; Plasma membrane calcium-transporting ATPase 2; Plasma membrane calcium-transporting ATPase 3; Plasma membrane calcium-transporting ATPase 4; PMCA1; PMCA2; PMCA3; PMCA4; sarcolemmal calcium pump; wriggle
Gene Aliases: 2810442I22Rik; ATP2B; ATP2B1; ATP2B2; ATP2B3; ATP2B4; CFAP39; CLA2; D6Abb2e; dfw; E130111D10Rik; Gena300; jog; MXRA1; OPCA; PMCA; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb; PMCA2; PMCA2a; PMCA2i; PMCA3; PMCA3a; PMCA4; PMCA4b; PMCA4x; SCAX1; Tmy; wms; wri
Entrez Gene ID: (Dog) 403480, (Sheep) 101111268, (Human) 490, (Chicken) 374244, (Rabbit) 100009543, (Human) 491, (Human) 492, (Human) 493, (Rat) 29598, (Mouse) 67972, (Rat) 24215, (Mouse) 11941, (Rat) 29599, (Rat) 29600
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