|Flow Cytometry (Flow)||1:50-1:200|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 100-200 of the human OCT4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is expected to react with swine, feline and bovine samples based on a 93% sequence homology.
Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NF-A3; Oct-3; Oct-4; OCT3; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; Octamer-binding transcription factor 3 antibody; OTF-3; OTF3 antibody.; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain class 5 transcription factor 1 antibody; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein
Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.