|ChIP assay (ChIP)||Assay Dependent|
|Flow Cytometry (Flow)||1µg per 10^6 cells|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2-5 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rabbit , Rat , Bovine , Mouse Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant PPAR alpha protein.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues.
MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates.
The MA1-822 immunogen is purified recombinant PPAR alpha protein.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; Peroxisome Proliferator Activated Receptor Alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha
Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha
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