|Flow Cytometry (Flow)||1:20|
|Western Blot (WB)||1:500-1:1500|
|Immunocytochemistry (ICC)||See 2 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Western Blot (WB)||See 6 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig , Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(284) M G E D K I K F K H I T P L(298) of mouse PPAR gamma 2.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits.
PA3-821A has been successfully used in IF and Western blot procedures. Previous batches of this antibody have been used in gel shift, immunocytochemistry, and immunofluoresence procedures. By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches gives predominant nuclear staining.
The PA3-821A immunizing peptide corresponds to amino acid residues 284-298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1, but exhibits no significant homology with PPAR alpha or NUC1. This sequence is completely conserved in mouse, human, and rat.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear
hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins
that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY- 14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Nuclear receptor subfamily 1 group C member 3; Peroxisome proliferator; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR-gamma
Gene Aliases: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2
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