|Western Blot (WB)||1:500|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant mouse PREB.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-610 detects recombinant mouse PREB (prolactin regulatory element binding protein).
PA1-610 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~45 kDa protein representing recombinant mouse PREB.
The PA1-610 antigen is recombinant mouse PREB.
Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites and these sites have been implicated in both basal level and kinase-mediated gene expression. The most proximal of these binding sites, termed 1P, has been shown to direct a response to numerous signals, such as cAMP and various G-proteins.
A novel protein, termed PREB (prolactin regulatory element binding protein), has been recently identified that regulates PRL gene expression through the 1P site, though it contains no discernable DNA-binding motif. Recent studies suggest that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests that PREB is a novel transcription factor that assists in PRL expression whether alone, or in concert with Pit-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Mammalian guanine nucleotide exchange factor mSec12; Prolactin Regulatory Element Binding Protein; Prolactin regulatory element-binding protein
Gene Aliases: C85705; Preb; Sec12
UniProt ID: (Mouse) Q9WUQ2
Entrez Gene ID: (Mouse) 50907
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