|Flow Cytometry (Flow)||2 ug/test|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2,000|
|Immunocytochemistry (ICC)||See 77 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 7 publications below|
|Western Blot (WB)||See 50 publications below|
|Immunoprecipitation (IP)||See 8 publications below|
|Immunohistochemistry (IHC)||See 38 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , C. elegans , Non-human primate , Mouse , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant rat PSD-95.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-045 has been successfully used in immunofluorescence analysis of PSD95 in human motor neurons derived from iPSCs.
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain.
PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: discs large homolog 4; Disks large homolog 4; Post Synaptic Density; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; PSD95; SAP-90; Synapse Associated Protein (SAP90); Synapse Associated Protein 95 kDa; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15
Gene Aliases: DLG4; Dlgh4; PSD-95; PSD95; SAP-90; SAP90; SAP90A
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.