|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 107 and 386 of Human PSMC4|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-30132 targets PSMC4 in IF, IHC(P) and WB applications and shows reactivity with Human samples.
The PA5-30132 immunogen is recombinant fragment corresponding to a region within amino acids 107 and 386 of Human PSMC4.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with an orphan member of the nuclear hormone receptor superfamily highly expressed in liver, and with gankyrin, a liver oncoprotein. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 26S protease regulatory subunit 6B; 26S proteasome AAA-ATPase subunit RPT3; MB67 interacting protein; MB67-interacting protein; MIP224; protease 26S subunit 6; proteasome (prosome, macropain) 26S subunit, ATPase, 4; Proteasome 26S subunit ATPase 4; S6; Tat-binding protein 7; TBP-7; TBP7
Gene Aliases: MIP224; PSMC4; RPT3; S6; TBP-7; TBP7
UniProt ID: (Human) P43686
Entrez Gene ID: (Human) 5704
Molecular Function: hydrolase
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