|Flow Cytometry (Flow)||2-3 ug/test|
|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||4-6 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 7-20 of P61289.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with canine, chicken, equine, orangutan, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PA28 alpha and PA28 beta are located primarily in the cytoplasm, whereas PA28 gamma is located in the nucleus. PA28 binds to the outer rings on both ends of the 20S proteasome to form a football-like structure. Binding of PA28 greatly stimulates multiple peptidase activities of the 20S proteasome in an ATP-independent reaction, but lacks the ability to degrade large protein substrates, suggesting that PA28 may cooperate with the 26 S proteasome in a sequential proteolytic pathway. In addition to its nuclear localization, PA28 gamma differs from PA28 alpha and PA28 beta in that it is not responsive to stimulation with IFN- gamma. Experiments with mice lacking the PA28 gamma gene core protein, which targets HCV for degradation, and with MEKK3, which phosphorylates PA28 gamma and increases its cellular levels. PA28 gamma also acts to increase resistance to apoptosis by promoting MDM2-mediated degradation of p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28g; PA28gamma; proteaseome (prosome, macropain) 28 subunit, 3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; REG gamma-3; REG-gamma
Gene Aliases: AA410043; AU020960; HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA; REGgamma
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