|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 130 and 193 of PUMA (Uniprot ID#Q9BXH1)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bcl-2-binding component 3; JFY-1; JFY1; p53 up-regulated modulator of apoptosis
Gene Aliases: BBC3; JFY-1; JFY1; PUMA
UniProt ID: (Human) Q96PG8
Entrez Gene ID: (Human) 27113
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