|Western Blot (WB)||2-5 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 36-53 of PUMA-delta and 196-213 of PUMA-gamma.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Some cross reactivity is seen with PUMA alpha.
This sequence is 59% homologus to PUMA alpha.
Suggested positive control: HeLa, NIH-3T3 cell lysate.
A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. PUMA-g is a novel member of the seven transmembrane spanning superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bcl-2 binding component 3; Bcl-2-binding component 3; BH3-only; FLJ42994; JFY-1; JFY1; p53 up-regulated modulator of apoptosis; PUMA
Gene Aliases: BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1
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