|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Guinea pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R I L G E E Q Y N R Y Q Q Y G A E E C of human Parkin.|
|Storage buffer||whole serum|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-18265 detects Parkin from rat and Guinea Pig samples.
PA1-18265 has been successfully used in immunohistochemistry, Western blot, and ELISA procedures.
The PA1-18265 immunogen is a synthetic peptide corresponding to residues R I L G E E Q Y N R Y Q Q Y G A E E C of human Parkin, conjugated to diphtheria toxoid.
Reconstitute with 100 ul of distilled water. Glycerol (1:1) may be added for additional stability.
Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E3 ubiquitin-protein ligase parkin; Park2; Parkin RBR E3 ubiquitin-protein ligase; parkin variant SV5DEL; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson protein 2, E3 ubiquitin protein ligase; Prkn; Ubiquitin E3 ligase PRKN
Gene Aliases: Park; Park2; Prkn
UniProt ID: (Rat) Q9JK66
Entrez Gene ID: (Rat) 56816
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