|Immunohistochemistry (Paraffin) (IHC (P))||1:30|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human parkin protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Alzheimer's brain tissue.
Parkin has been mapped to chromosome 6 and encodes a protein of 465 amino acids with similarity to ubiquitin at the amino terminus and a RING-finger motif at the carboxyl terminus. Parkinson and quote;s disease is the second most common neurodegenerative disease following Alzheimer and quote;s. The characteristic movement disorder results from dysfunction and neuron loss in the substantia nigra. Deletions in parkin, have been associated with autosomal-recessive juvenile parkinsonism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E3 ubiquitin-protein ligase parkin; PARK2; Parkin; Parkin RBR E3 ubiquitin-protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin)
Gene Aliases: AR-JP; LPRS2; PARK2; PDJ; PRKN
UniProt ID: (Human) O60260
Entrez Gene ID: (Human) 5071
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