|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
|Immunocytochemistry (ICC)||See 3 publications below|
|Western Blot (WB)||See 2 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog , Rat , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified recombinant full-length human paxillin protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls include HeLa cells and colon carcinoma.
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: AW108311; AW123232; Pax; PXN
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