|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1:1000-1:4000|
|Western Blot (WB)||See 6 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1051 detects perilipin A from rat and 3T3-L1 cell extract.
PA1-1051 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~62 kDa protein representing perilipin A from 3T3-L1 cell extract
The PA1-1051 immunogen is a synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A. This sequence is 93% conserved in humans. The PA1-1051 immunizing peptide (Cat. # PEP-169) is available for use in neutralization and control experiments.
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body.
Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Lipid droplet-associated protein; PERI; PERIA; Perilipin A; perilipin B; Perilipin-1; Plin1
Gene Aliases: 6030432J05Rik; Peri; PERIA; Plin; Plin1
UniProt ID: (Mouse) Q8CGN5
Molecular Function: transfer/carrier protein
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