|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Rat Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1052 detects perilipin A and B from rat and 3T3-L1 cell extract.
PA1-1052 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~62 and 46 kDa proteins representing perilipin A and B, respectively from 3T3-L1 cell extract
The PA1-1052 immunogen is a synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B. This sequence is 82% conserved in humans. The PA1-1052 immunizing peptide (Cat. # PEP-170) is available for use in neutralization and control experiments.
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body.
Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADFP; Adipophilin; adipose differentiation related protein; Adipose differentiation-related protein; ADRP; Lipid droplet-associated protein; PERI; PERIA; Perilipin A; perilipin B; Perilipin-1; Perilipin-2; PLIN; Plin1; Plin2
Gene Aliases: 6030432J05Rik; AA407157; Adfp; ADPH; Adrp; Peri; PERIA; Plin; Plin1; Plin2
Molecular Function: transfer/carrier protein
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.