|Flow Cytometry (Flow)||1:20|
|Western Blot (WB)||1:1000-1:10000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified human recombinant Peroxiredoxin 3.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-752 detects Peroxiredoxin 3 protein in human samples. This antibody is specific for Prx 3 and shows no cross-reactivity with other Prx isoforms.
PA3-752 has successfully been used in Western blot, and immunocytochemistry procedures. By Western blot, this antibody detects an ~27 kDa protein representing Prx 3 in human PC3 cell lysate.
The PA3-752 antigen is purified human recombinant Prx 3 from HEK 293 cells.
The peroxiredoxin (Prdx, Prx, or Trx-Px) family of enzymes is a recently identified family of peroxidases found in free-living organisms. The six known isoforms (Prx1-6) play an important role in protecting lipids, enzymes and DNA against peroxides, such as hydrogen peroxide. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. This is acomplished by the active cysteine of Prx reducing peroxides, which is then converted into a transient cysteine sulfenic acid or cysteine sulfinic acid. If Prx protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer and quote;s or DNA damage leading to cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Peroxiredoxin-3; Protein MER5 homolog; Prx 3; Prx-III; Thioredoxin-dependent peroxide reductase, mitochondrial
Gene Aliases: AOP-1; AOP1; HBC189; MER5; PRDX3; PRO1748; prx-III; SP-22
UniProt ID: (Human) P30048
Entrez Gene ID: (Human) 10935
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