|Western Blot (WB)||1:2000|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sulfonylated peptide (KLH coupled) corresponding to the active site sequence common to mammalian Prx 6.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is HeLa cells treated with H2O2.
Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at their active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Prx I to IV have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Prx I. The active site cysteine (Cys51) is oxidized to cysteine sulfenic acid (Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress condition, the sulfenic intermediate (Cys51-SOH) can be easily overoxidized to cysteine sulfinic acid (Cys-SO2H) or cysteine sulfonic acid (Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that overoxidized Prx can be reduced back to the active form during recovery after oxidative stress. Anti-Prx-SO3 antibody recognizes both sulfinic and sulfonic forms of Prx and detects overoxidized Prx enzymes in H2O2-treated cells with high sensitivity and specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; epididymis secretory sperm binding protein Li 128m; Liver 2D page spot 40; MSP23; natural killer-enhancing factor A; NKEFA; Non-selenium glutathione peroxidase; NSGPx; PAGA; PAGB; Peroxiredoxin-6; Red blood cells page spot 12
Gene Aliases: 1-Cys; aiPLA2; AOP2; HEL-S-128m; KIAA0106; NSGPx; p29; PRDX6; PRX
UniProt ID: (Human) P30041
Entrez Gene ID: (Human) 9588
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