|ChIP assay (ChIP)||Assay-Dependent|
|Immunocytochemistry (ICC)||2 ug/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide to a region of human AKT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-20325 detects phospho-AKT1 (pS473) in human and mouse samples.
MA1-20325 has been successfully used in immunofluorescence, ChIP, immunoprecipitation, and Western blot procedures.
The MA1-20325 immunogen is a synthetic peptide corresponding to a region of human AKT1
The Akt family of serine/threonine protein kinases (also called protein kinase B/PKB) plays a key role in insulin signaling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT; AKT1 kinase; AKT1m; Akt1m protein; MGC99656; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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