|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human AKT1/3 around the phosphorylation site of Tyr437 (T-R-YP-F-D)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT1; AKT1 kinase; AKT1m; Akt1m protein; AKT3; AKT3 kinase; murine thymoma viral (v-akt) oncogene homolog 1; PKB; PKB alpha; PKB gamma; PKBG; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B gamma; protein kinase B, gamma; protein kinase B-alpha; Proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC-alpha serine/threonine-protein kinase; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AI851531; AKT; AKT1; AKT3; CWS6; D930002M15Rik; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBG; PRKBA; PRKBG; RAC; RAC-ALPHA; RAC-gamma; RAC-PK-gamma; STK-2
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.