|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
AF-6, also known as afadin, is expressed in a variety of cell types. At least 5 isoforms of this protein are produced by alternative splicing and the 'canonical' sequence is designated as l-afadin. Chromosomal aberration of the MLLT4 gene and tight junctions and Notch signaling pathways cross-communication. This happens through physical interaction with Ras, Notch and Dishevelled, a key Wingless effector. AF-6 also controls integrin-mediated cell adhesion. This is done at least partially by regulating Rap1 activation through recruitment of both SPA-1 and Rap1GTP. These results demonstrate that AF-6 is an important molecule mediating cell-cell adhesion but is also capable of modulating several key signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Afadin; Afadin adherens junction formation factor; ALL1-fused gene from chromosome 6 protein; MLLT4; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; Protein AF-6
Gene Aliases: AF6; AFDN; MLL-AF6; MLLT4
UniProt ID: (Human) P55196
Entrez Gene ID: (Human) 4301
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