|Western Blot (WB)||1:1000|
|Tested Species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 of rat AQP2 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
This antibody is predicted to react with canine, bovine, chicken, human, mouse and non-human primate based on 100% sequence homology.
PA5-35387 is specific for the ~29kDa AQP2 protein phosphorylated at Ser264, and also recognizes the glycosylated form of AQP2 at ~37kDa. This antibody contains enough material to conduct 10 mini-Western blots.
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADH water channel; AQP-2; AQP-CD; Aquaporin-2; Aquaporin-CD; Collecting duct water channel protein; Water channel protein for renal collecting duct; water-channel aquaporin 2; WCH-CD
Gene Aliases: AQP-2; Aqp2; aquaporin-2
UniProt ID: (Rat) P34080
Entrez Gene ID: (Rat) 25386
Molecular Function: transporter
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