|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Artemis around the phosphorylation site of Serine 516|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 78 kDa.
Purity is >95% by SDS-PAGE.
Distinct DNA repair pathways minimize the consequences of mutagenic events. Reactive oxygen species (ROS) are highly reactive atoms with an unpaired electron that are conducive to double-strand DNA breaking events. Artemis, named after the Greek goddess for the protection of children, is one of the major proteins contributing to the preservation of double-strand breaks in DNA by cutting away the damaged parts of the DNA, which allows the strands to rejoin. Artemis is a single-strand-specific 5' to 3' exonuclease that forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs). DNA-PKcs phosphorylates Artemis, and Artemis acquires endonucleolytic activity on 5' and 3' overhangs and hairpins. These activities are essential for V(D)J recombination and for the 5' and 3' overhang processing in nonhomologous DNA end joining.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARTEMIS; ASCID; DCLRE1C; DNA cross-link repair 1A, PSO2 homolog; DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae); DNA cross-link repair 1C protein; hSNM1C; mArt; Protein A-SCID; Protein artemis; SCIDA; severe combined immunodeficiency, type a (Athabascan); SNM1 homolog C; SNM1-like protein; SNM1C
Gene Aliases: 9930121L06Rik; A-SCID; AI661365; Art; ARTEMIS; ASCID; DCLRE1C; DCLREC1C; RS-SCID; SCIDA; SNM1C; Snm1l
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