|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 807 (G-D-Y(p)-E-N) derived from Human BL-CAM.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is COS7 cells.
CD22 is a transmembrane glycoprotein of B lymphocytes. CD22 is expressed in the cytoplasm of immature B cells and in the membrane of mature B cells. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin and quote;s lymphoma, but negative in classical Hodgkin and quote;s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; CD22 antigen; FLJ22814; MGC130020; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; SIGLEC-2; SIGLEC2; T-cell surface antigen Leu-14
Gene Aliases: CD22; SIGLEC-2; SIGLEC2
UniProt ID: (Human) P20273
Entrez Gene ID: (Human) 933
Molecular Function: cell adhesion molecule cytokine receptor defense/immunity protein immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule membrane-bound signaling molecule myelin protein receptor signaling molecule structural protein
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