|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for IHC is human brain tissue.
Required for lipopolysaccharide (LPS)-induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T helper cell differentiation and IFNG expression in T cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: augmented in rheumatoid arthritis 2; C-COT; Cancer Osaka thyroid oncogene; cancer Osaka thyroid, oncogene; cot (cancer Osaka thyroid) oncogene; COT proto-oncogene serine/threonine-protein kinase; Ewing sarcoma transformant; mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; serine/threonine kinase (Tpl-2); Serine/threonine-protein kinase cot; TPL-2; Tumor progression locus 2
Gene Aliases: AURA2; c-COT; COT; Cot/Tpl2; D17TPL2; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.