|Western Blot (WB)||1:250|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues K(123) R R E I L S R R P S(p) Y R K(136) of human CREB.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-851B detects the phosphorylated form of cyclic-AMP response element binding protein (CREB) from rat and human cells. This antibody does not detect the unphosphorylated form of CREB.
PA1-851B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~43 kDa protein representing phospho-CREB from forskolin treated PC12 cell lysate.
The PA1-851B immunogen is a synthetic phospeptide corresponding to residues K(123) R R E I L S R R P S(p) Y R K(136) of human CREB. This sequence is completely conserved between human, bovine, canine, mouse, pig, sheep, and rat CREB protein. The PA1-851B immunizing peptide (Cat. # PEP-143) is available for use in neutralization and control experiments.
Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; Phospho-Cyclic AMP Response Element Binding Protein; transactivator protein; Y protein
Gene Aliases: CREB; CREB-1; CREB1
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