|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human CaMK4 around the phosphorylation site of Threonine 200|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Ca2+/calmodulin-dependent protein kinase type IV/Gr; calcium/calmodulin-dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calmodulin-dependent protein kinase IV; calspermin; CAM kinase IV; CAM kinase- GR; CAM kinase-GR; CaMK IV; CAMK4; CaMKIV; EC 18.104.22.168; KCC4; kinase CaMK4
Gene Aliases: A430110E23Rik; AI666733; caMK; CaMK IV; CaMK-GR; CaMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; IV; RATCCDPK
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.