|Flow Cytometry (Flow)||1:20|
|Western Blot (WB)||1:250|
|Western Blot (WB)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||All , Tag , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse cortactin that contains tyrosine 421.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1110020L01Rik; Amplaxin; cortactin; CTTN; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); FLJ34459; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); Oncogene EMS1; Src substrate cortactin; SRC8
Gene Aliases: 1110020L01Rik; CTTN; EMS1
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