|Flow Cytometry (Flow)||1-3 µg/10^6 cells|
|Immunofluorescence (IF)||1-2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:1000|
|Western Blot (WB)||1:500-1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 390-401 of Q05397.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse, rat, primate, bovine, equine, canine, chicken, opossum, zebra finch, zebrafish, Xenopus and pufferfish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FADK 1; FAK-related non-kinase polypeptide; Focal Adhesion Kinase; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2
Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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