|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sythetic peptide corresponding to amino acid residues surrounding phospho-Ser845 of rat GluR1|
|Storage buffer||HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody recognizes a ~100kD band corresponding to GluR1 phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling can blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide, and is completely eliminated by treatment with lambda-phosphatase.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GluR-1; GluR-A; GluR-K1; GluR1; GluRA; Glutamate Receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GRIA1; HGBR1
Gene Aliases: 2900051M01Rik; Glr-1; Glr1; GluA1; GLUH1; Glur-1; GluR-A; gluR-K1; GLUR1; GLURA; GRIA1; HBGR1; HIPA1
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