|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Mouse, Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human SAPK/JNK around the phosphorylation site of Tyrosine 185|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-Jun N-terminal kinase 1; FLJ12099; FLJ33785; JNK1 beta1 protein kinase; JNK3; JNK3 alpha protein kinase; JNK3A; JUN N-terminal kinase; MAP kinase 10; MAP kinase 8; MAPK 10; MAPK 8; MAPK10; MGC50974; mitogen activated protein kinase 8; Mitogen-activated protein kinase 10; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; MK10; p493F12; p54bSAPK; PRKM10; protein kinase mitogen-activated 8; stress activated protein kinase bet; stress-activated protein kinase 1; stress-activated protein kinase 1c; stress-activated protein kinase JNK1
Gene Aliases: AI849689; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c
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