|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of threonine 1079/1041 (E-F-T(p)-F-R) derived from Human LATS1/2 .|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for IHC is human brain tissue.
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
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Protein Aliases: Drosophila) homolog; h-warts; Kinase phosphorylated during mitosis protein; large tumor suppressor homolog; Large tumor suppressor homolog 1; Large tumor suppressor homolog 2; large tumor supressor, homolog 1; LATS (large tumor suppressor; LATS (large tumor suppressor, Drosophila) homolog 1; LATS (large tumor suppressor, Drosophila) homolog 2; LATS, large tumor suppressor, homolog 1; LATS, large tumor suppressor, homolog 2; LATS1; LATS2; serine/threonine kinase KPM; Serine/threonine-protein kinase kpm; serine/threonine-protein kinase LATS; Serine/threonine-protein kinase LATS1; Serine/threonine-protein kinase LATS2; WARTS protein kinase; Warts-like kinase
Gene Aliases: 4932411G09Rik; AV277261; AW208599; AW228608; KPM; LATS1; LATS2; WARTS; wts
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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