|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 394 (N-E-Y(p)-T-A) derived from Human Lck.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is JK cells.
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
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Protein Aliases: EC 220.127.116.11; kinase Lck; LCK; Leukocyte C-terminal Src kinase; LSK; LSK-T; Lymphocyte cell-specific protein-tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; P56-LCK; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Lck
Gene Aliases: Hck-3; IMD22; LCK; LSK; Lsk-t; Lskt; p56<lck>; p56Lck; pp58lck; YT16
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