|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine, chicken, human, mouse, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots of rat brain homogenates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by Protein Kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996). MARCKS is a member of a family of calmodulin binding protein and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 156); myristoylated alanine-rich C-kinase substrate; Myristoylated alanine-rich protein kinase C substrate; phospho-Myristoylated Alanine-Rich C Kinase Substrate (Ser152; protein kinase C substrate 80 kDa protein
Gene Aliases: KINC; Macs
Entrez Gene ID: (Rat) 25603
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