|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is MCF-7 cells; suggested positive control for IHC is human lung carcinoma.
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
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Protein Aliases: 80K-L; 80K-L protein; FLJ14368; FLJ90045; MACS; MARCKS; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); phosphomyristin; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain
Gene Aliases: 80K-L; MACS; MARCKS; PKCSL; PRKCSL
UniProt ID: (Human) P29966
Entrez Gene ID: (Human) 4082
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