|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 of human MAP2K1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine, canine, mouse, non-human primate and rat based on 100% sequence homology.
This antibody is specific for the ~45 kDa MEK 1 protein phosphorylated at Thr292 in Western blots of human brain extracts.
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993;Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adamsand Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MKK (Thr292); phospho-MAP Kinase Kinase; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
UniProt ID: (Human) Q02750
Entrez Gene ID: (Human) 5604
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