|Flow Cytometry (FACS)||1:50|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1,000|
|Tested Species reactivity||Mouse, Human, Rat, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr286 of human MEK1.|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl|
MAP2K1 is a dual specificity mitogen-activated protein kinase (MAPK) kinase. It is an essential component of the MAP kinase pathway and stimulates the enzymatic activity of MAP kinases in response to various signals. Growth factors, cytokines, and proto-oncogenes transduce their signals through the activation of RAS, which leads to activation of the serine/threonine kinase RAF, and then to activation of MAPK kinase. MAP2K1 is activated by the Raf family member p74raf-1, through phosphorylation of serine residues at positions 217 and 221 in the activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1
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