|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of serine 80 (T-H-S(p)-I-E) derived from Human SEK1/MKK4.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
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Protein Aliases: C-JUN N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK 1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MEK 4; mitogen activated protein kinase kinase 4, variant 1; SAPK kinase 1; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; SAPKK-1; SAPKK1; SEK1; Stress-activated protein kinase kinase 1
Gene Aliases: JNKK; JNKK1; MAP2K4; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1
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