|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of Serine 2409(Q-D-S(p)-E-D) derived from Human IGF2R.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is COS7 cells; suggested positive control for IHC is human brain tissue.
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 300 kDa mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor; Cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI Man-6-P receptor; CI-MPR; CIMPR; IGF-II receptor; IGF-II/CI-MPR; IGF2R; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P-R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose-6-phosphate receptor, cation independent; MPR 300; MPR1; MPRI
Gene Aliases: AI661837; CD222; CI-M6PR; CI-MPR; CIMPR; IGF2R; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI
Molecular Function: receptor
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