|Western Blot (WB)||1:500-1:2000|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human MITF around the phosphorylation site of Serine 180/73|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
This antibody recognizes MiTF phosphorylated at Ser180 as well as Ser73 (M1 and M2 isoforms).
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: black eyed white; class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; Microphthalmia-associated transcription factor; MITF M1
Gene Aliases: BCC2; Bhlhe32; bw; CMM8; Gsfbcc2; MI; vit; Vitiligo; Wh; WS2; WS2A
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