|ELISA (ELISA)||1:5000 - 1:20,000|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:5000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues K(12) K R P Q R A T (pS) N V F A M F D(27) of the human Myosin Light Chain protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-26470 detects Myosin light chain (phospho S20) from human samples. PA1-26470 is expected to cross react with all mammals and birds due to 100% sequence homology.
PA1-26470 has been successfully used in Western blot, IF, ICC, IHC (P) and ELISA procedures.
The PA1-26470 immunogen is a synthetic phosphopeptide corresponding to residues K(12) K R P Q R A T (pS) N V F A M F D(27) of the human Myosin Light Chain protein.
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A1 catalytic; A2 catalytic; MLC1/MLC3; MLC1F; MLC1F/MLC3F; MLC3F; Myosin light chain 1/3, skeletal muscle isoform; Myosin light chain A1/A2; Myosin light chain alkali 1/2; myosin light chain, alkali, fast skeletal muscle; myosin, light chain 1, alkali; skeletal, fast; myosin, light polypeptide 1, alkali; atrial, embryonic; myosin, light polypeptide 1, alkali; skeletal, fast; myosin, light polypeptide 4, alkali; atrial, embryonic
Gene Aliases: AI325107; MLC1F; MLC3F; MYL1; Mylf
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