|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human NIPA around the phosphorylation site of Ser354 (T-R-SP-W-D)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1110054L24Rik; hematopoietic stem/progenitor cell protein 216; hNIPA; mNIPA; NIPA; nuclear interacting partner of ALK; nuclear interacting partner of anaplastic lymphoma kinase (ALK); nuclear interaction partner of ALK; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; zinc finge; Zinc finger C3HC-type protein 1; zinc finger, C3HC-type containing 1
Gene Aliases: 1110054L24Rik; AU018540; AU019789; HSPC216; NIPA; ZC3HC1
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