|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 of rat GRIN2B conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with human, mouse and non-human primate based on 100% sequence homology.
This antibody is specific for ~180 kDa NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling can be blocked by lambda-phosphatase treatment.
The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, NMDA2B; hNR3; N methyl D aspartate receptor subtype 2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B
Gene Aliases: GluN2B; Grin2b
UniProt ID: (Rat) Q00960
Entrez Gene ID: (Rat) 24410
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