|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of rat GRIN2B conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20°C or -80°C if preferred|
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects a ~180 kDa protein representing NMDAR NR2B-subunit protein phosphorylated at Tyr1472. It also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either lamda-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; Glutamate receptor ionotropic NMDA2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, NMDA2B; GRIN2B; hNR 3; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B
Gene Aliases: GluN2B; Grin2b
UniProt ID: (Rat) Q00960
Entrez Gene ID: (Rat) 24410
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